TABLE 1.

Mutations repeatedly observed in cultures of strain PA77^a

Gene	Locus tag		Mutation(s) in:
	PAO1	PA77	v01	v02	v03	v04	v05	v05a	v08a	v10a	v11a
pmrB	PA4777	PSA77_03611	V9A, L17Q	L90Q, E320K	V9A	P216Q	P254L	P169X, M292I	S257N	N41I, P169X	H261Y
pmrE	PA2043	PSA77_06074	Y28N	Y28C	Y28N	Y28C	Y28N	Y28C	Y28C	Y28N	Y28N
lptD	PA3559	PSA77_05098						Y803X			L538R

^aVials v01 to v05 refer to the experiment discussed in the text; v05a, v08a, v10a, v11a stem from the preliminary experiment with strain PA77, which ran for only 15 days. The full list including annotation of each mutation is available in Data Set S3 in the supplemental material.