TABLE 2.

Mutations repeatedly observed in cultures of strain PA83^a

Gene	Locus tag		Mutation(s) in:
	PAO1	PA83	v02	v03	v05	v06	v08	v11	v12	v14	v15
lpxC	PA4406	PSMA83_05042	P101S	V222A, S106G	V222A	V164G, A107T	A107T, G21W, F176S	A107T, I131F	M103I	D232E, D232G, V217F, V217A	V222A, S106G
pmrB	PA4777	PSMA83_05449	L96R	L171P	L87P	F51L	S8P, E320K	V9A	G123S	E320K, A248T, L167P	R259H, V361M
Gene for putative transferase	PA3853	PSMA83_01172	C226G	Y3C, G62S	V34A, Y155C		C226G	R60C, Y216C, E185G	C226G		V122A, E185G
Gene for asparagine synthetase		PSMA83_05677	L365P	Frameshift	L425P			G32S	Frameshift	W153X	L365P, W153X V286M
migA	PA0705	PSMA83_04868	H219P	C25R, N27S			D106G	Q191R, V22A	T196P, H123P	H219P	A168T
mutS	PA3620	PSMA83_01420		T51P	T51P	T51P		T51P		T51P	T51P, T287P
lpxO2	PA0936	PSMA83_04590	D163A	D163N	W209X		D163A		Frameshift		In-frame deletion
pmrA	PA4776	PSMA83_05448	L11Q				L11P		R159L, G15V, N172D
Gene for putative outer membrane protein	PA3647	PSMA83_01392		K122X					Frameshift		V1V, R2G
Gene for paraquat-inducible protein		PSMA83_05349			G180X				Frameshift
1	PA4773	PSMA83_05445			W35X					Frameshift
cupB5	PA4082	PSMA83_00934		G260X, R26C		P139P
2	PA5133	PSMA83_05845				Frameshift		Frameshift			Frameshift
pdtA	PA0690	PSMA83_04884					A3885V, A3885A	G1527X
morA	PA4601	PSMA83_05248	R1199H								G143D
lpxA	PA3644	PSMA83_01395	R96S								R191C
priA	PA5050	PSMA83_05733		L38L				R689R
Gene for putative chemotaxis transducer	PA4633	PSMA83_05281		L315P	A260A
Gene for putative lipoprotein	PA3069	PSMA83_01992			K83R	L68P
traN		PSMA83_06409		W773X	G912D
Gene for polyketide synthase type I		PSMA83_02547			I876M			V781V
3	PA0242	PSMA83_00250				S420G		A579A
Gene for hypothetical protein	PA4782	PSMA83_05455				P38S		P38P
wbpM	PA3141	PSMA83_01912	E273K			E273G
mscL	PA4614	PSMA83_05261						V86I			S35P

^aThe complete list of mutations, including annotation and locus tag of each gene, is available in Data Set S4. For genes with long names, the table lists the PAO1 locus tag where available; the annotations of these genes are as follows: 1, putative S-adenosylmethionine decarboxylase proenzyme; 2, putative membrane-bound metallopeptidase; and 3, putative 4-hydroxyphenylpyruvate dioxygenase.