FIG 7.

In vivo efficacy of tomatidine in a mouse model of systemic infection. (A) Schematic representation of the infection and treatment protocol. Mice were infected with 4 × 10⁵ CFU through the tail vein. Tomatidine treatment (50 mg/kg) (or placebo) was administered intraperitoneally at 7, 24, and 31 h postinfection (p.i.). At 48 h p.i., animals were sacrificed and kidneys were collected for determination of the number of CFU. (B) Fungal burden in tomatidine-treated mice. The number of CFU per gram of kidney is plotted for placebo- and tomatidine-treated mice. Significant differences in the distribution of the number of CFU were assessed using the Mann-Whitney test (n = 9 and 10 for the tomatidine- and placebo-treated mice, respectively; P = 0.031).