FIG 1.

Inhibitory effect of rilpivirine on efflux of Hoechst 33342 (80 μM) from MDCK-MDR1 (A), MDCK-BCRP (E), and MDCK-PAR (B and F) cell lines; efflux of rhodamine 123 (10 μM) from MDCK-MDR1 (C) and MDCK-PAR (D) cells; and efflux of calcein AM (0.25 μM) from MDCK-MRP2 (G) and MDCK-PAR (H) cell lines. LY335979 (1 μM), Ko143 (2.5 μM), and MK-571 (50 μM), the model inhibitors of MDR1, BCRP, and MRP2, respectively, were used as positive controls. Data are shown as mean values ± SD from at least three experiments performed in triplicate. Statistical significance was analyzed by one-way ANOVA followed by Bonferroni's test. **, P ≤ 0.01; ***, P ≤ 0.001.