TABLE 1.
Characterization of fosA3-positive E. coli isolates and their fosA3-carrying plasmids
| Isolatea | Host | MLST | Phylogroup | Other cotransferred resistance genesb | Context of fosA3 |
fosA3-carrying plasmids |
|||
|---|---|---|---|---|---|---|---|---|---|
| Size | Replicon type | EcoRI RFLPc | Addiction system(s) | ||||||
| SY301B | Pig | ST761 | B2 | blaCTX-M-65, floR, cfr, tet(A) | III | pECXH3 (∼97 kb) | N (ST7) | A1 | pemKI, hok-mok-sok, srnB-srnC |
| SY301F | Pig | ST3315 | B2 | blaCTX-M-55, floR, cfr, rmtB, strA, strB, tet(A) | I | ∼105 kb | N (ST7) | A2 | pemKI, hok-mok-sok, srnB-srnC |
| SY303P | Pig | ST5714 | B2 | blaCTX-M-65, floR, rmtB, tet(A) | III | ∼100 kb | N-F33:A−:B− | NT | pemKI, hok-mok-sok, srnB-srnC |
| SY301L | Pig | ST5442 | B2 | blaCTX-M-55, floR, oqxA, oqxB, blaTEM-1, tet(A) | II | ∼97 kb | F33:A−:B− | C1 | pemKI, hok-mok-sok, srnB-srnC |
| SY303M | Pig | ST3944 | B2 | blaCTX-M-14, floR, cfr, rmtB, aadA2, blaTEM-1 | IV | ∼97 kb | N | A1 | pemKI, hok-mok-sok, srnB-srnC |
| SY305M | Pig | ST3944 | B2 | blaCTX-M-14, floR, cfr, rmtB, aadA2, blaTEM-1 | IV | ∼97 kb | N | A1 | pemKI, hok-mok-sok, srnB-srnC |
| SY286M | Cow | ST93 | B2 | blaCTX-M-14, rmtB, aadA2, blaTEM-1 | IV | pECM13 (113,006 bp) | IncI1 (ST136) | E1 | pemKI, hok-mok-sok |
| SY287M | Cow | ST98 | B2 | blaCTX-M-14, floR, rmtB, aadA2, blaTEM-1 | IV | pECM13-like | IncI1 (ST136) | E1 | pemKI, hok-mok-sok |
| SY284B | Cow | ST4683 | B1 | blaCTX-M-14, floR, rmtB, aadA2, blaTEM-1 | IV | pECM13-like | IncI1 (ST136) | E1 | pemKI, hok-mok-sok |
| CH293B | Chicken | ST10 | B2 | blaCTX-M-55, floR, strA, strB, tet(A) | I | ∼97 kb | F33:A−:B− | C1 | pemKI, hok-mok-sok, srnB-srnC |
| CH292B | Chicken | ST10 | B2 | blaCTX-M-55, floR, cfr, blaTEM-1, strA, strB, tet(A) | II | pECB11 (92,545 bp) | F33:A−:B− | C2 | pemKI, hok-mok-sok, srnB-srnC |
| CH291M | Chicken | ST410 | B2 | blaCTX-M-65, floR, rmtB | III | ∼100 kb | N-F33:A−:B− | C3 | pemKI, hok-mok-sok, srnB-srnC |
| CH281F | Chicken | ST5889 | A | blaCTX-M-65, floR, rmtB | III | ∼100 kb | N (ST7) | A2 | pemKI, hok-mok-sok, srnB-srnC |
| CH282M | Chicken | ST1437 | A | blaCTX-M-65, floR, rmtB, oqxA, oqxB | III | ∼100 kb | N (ST7) | A2 | pemKI, hok-mok-sok, srnB-srnC |
| CH285F | Chicken | ST448 | B2 | blaCTX-M-3, floR, strA, strB, tet(A) | I | ∼105 kb | F14:A−:B− | NT | pemKI, hok-mok-sok |
| DH286F | Chicken | ST2518 | B2 | blaCTX-M-3, rmtB, strA, strB, tet(A) | I | pECF12 (77,822 bp) | F33:A−:B− | D1 | pemKI |
| DH286M | Chicken | ST617 | B2 | blaCTX-M-55, floR, blaTEM-1, strA, strB, tet(A) | II | ∼120 kb | F33:A−:B− | NT | pemKI, hok-mok-sok, srnB-srnC |
| JL12G | Cow | ST559 | B2 | blaCTX-M-55, floR, blaTEM-1, strA, strB, tet(A) | II | pECB11-like | F33:A−:B− | C2 | pemKI, hok-mok-sok, srnB-srnC |
| JL15P | Cow | ST209 | B2 | blaCTX-M-55, floR, blaTEM-1, strA, strB, tet(A) | II | pECB11-like | F33:A−:B− | C2 | pemKI, hok-mok-sok, srnB-srnC |
| JL17P | Cow | ST359 | A | blaCTX-M-65, tet(A) | III | ∼100 kb | N (ST7) | NT | pemKI, hok-mok-sok, srnB-srnC |
| JT14G | Cow | ST5689 | B1 | blaCTX-M-14, floR, rmtB, aadA2, blaTEM-1 | IV | ∼95 kb | N | A3 | pemKI, hok-mok-sok, srnB-srnC |
| JT14P | Cow | ST5714 | B1 | blaCTX-M-14, floR, rmtB, aadA2, blaTEM-1 | IV | ∼95 kb | N | A4 | pemKI, hok-mok-sok, srnB-srnC |
| HL12L | Cow | ST195 | D | blaCTX-M-55, floR, strA, strB, tet(A) | I | ∼97 kb | F33:A−:B− | C1 | pemKI, hok-mok-sok, srnB-srnC |
| HL36B | Cow | ST4680 | B1 | blaCTX-M-55, strA, strB, tet(A) | I | ∼100 kb | N | A3 | pemKI |
| HL40C | Farmer | ST4680 | B1 | blaCTX-M-14, floR, aadA2, blaTEM-1 | IV | ∼105 kb | IncI1 (ST136) | E2 | None |
| HB37B | Chicken | ST1725 | B1 | blaCTX-M-55, floR, rmtB, strA, strB, tet(A) | I | pECF12-like | F33:A−:B− | D1 | pemKI |
| HB35D | Chicken | ST48 | B2 | blaCTX-M-123 | III | ∼90 kb | F14:A−:B− | NT | pemKI, hok-mok-sok, srnB-srnC |
| HB38B | Cow | ST2055 | B1 | blaCTX-M-14, rmtB, aadA2, blaTEM-1 | IV | ∼140 kb | IncI1 (ST136) | E3 | pemKI |
| HB38L | Cow | ST5693 | B1 | blaCTX-M-14, rmtB, aadA2, blaTEM-1 | IV | ∼120 kb | IncI1 (ST136) | E4 | None |
| HB18F | Cow | ST1081 | B1 | blaCTX-M-14, floR, rmtB, aadA2, blaTEM-1 | IV | ∼140 kb | IncI1 (ST136) | E3 | pemKI |
| HB13B | Chicken | ST167 | B2 | blaCTX-M-55, rmtB, strA, strB, tet(A) | I | pECF12-like | F33:A−:B− | D2 | pemKI |
| HB13M | Cow | ST4463 | B2 | blaCTX-M-65, rmtB, tet(A) | III | ∼120 kb | N | NT | None |
| HB312G | Cow | ST685 | B2 | blaCTX-M-65, floR, rmtB, strA, strB, tet(A) | III | ∼120 kb | IncI1 (ST136) | E4 | None |
| SH312M | Pig | ST1488 | B2 | blaCTX-M-55, floR, blaTEM-1, strA, strB, tet(A) | II | pECB11-like | F33:A−:B− | C2 | pemKI, hok-mok-sok, srnB-srnC |
| SH312N | Pig | ST1313 | B2 | blaCTX-M-65, floR, oqxA, oqxB | III | ∼120 kb | N | B | pemKI, hok-mok-sok, srnB-srnC |
| SH21F | Pig | ST209 | B2 | blaCTX-M-55, floR, blaTEM-1, strA, strB, tet(A) | II | pECB11-like | F33:A−:B− | C2 | pemKI, hok-mok-sok, srnB-srnC |
| SH21G | Pig | ST354 | D | blaCTX-M-55, floR, cfr, blaTEM-1, strA, strB, tet(A) | II | pECB11-like | F33:A−:B− | C2 | pemKI, hok-mok-sok, srnB-srnC |
| SH21M | Pig | ST648 | D | blaCTX-M-55, floR, blaTEM-1, strA, strB, tet(A) | II | pECB11-like | F33:A−:B− | C2 | pemKI, hok-mok-sok, srnB-srnC |
| SH33L | Cow | ST3743 | B1 | blaCTX-M-65, tet(A) | III | ∼100 kb | IncI1 (ST136) | E5 | None |
a
The first two capital letters in each strain name represent the city in which the isolate was obtained: SY, Shenyang (Liaoning Province); CH, Changchun (Jilin Province); DL, Dehui (Jilin Province); JL, Jilin (Jilin Province); JT, Jiutai (Jilin Province); HL, Hailaer (Inner Mongolia autonomous region); HB, Harbin (Heilongjiang Province); SH, Suihua (Heilongjiang Province). Isolates from healthy animals are underlined.
b
Resistance genes were transferred by conjugation and determined by PCR.
c
RFLP patterns were assigned to the same major RFLP profiles (A–E) when they differed in ≤3 bands. The numbers (e.g., A1, A2 etc.) indicate minor variations within the RFLP patterns. NT, not typeable.