FIG 3.

Splenic parasite burden in infected susceptible BALB/c mice in the model of early CL. The number of viable L. major parasites in the spleens of treated mice was quantified by limiting dilution assay at weeks 3 (A), 6 (B), and 9 (C) postinfection with L. major promastigotes. AMB formulations were administered i.v. on days 1, 2, 4, 7, 14, 21, and 28 after parasite injection. ** (P < 0.01) and *** (P < 0.001) indicate the significant differences when comparing splenic parasite loads for Fungizone (A) or DSHemsPC-AmB-Lip and AmBisome (B) to empty liposome and 5% dextrose.