FIG 9.

Footpad parasite burden in infected susceptible BALB/c mice in a model of established CL. The number of viable L. major parasites in the footpads of treated mice was quantified by limiting dilution assay at weeks 3 (A), 6 (B), and 9 (C) postinfection with L. major promastigotes. AMB formulations were administered i.v. on days 23, 25, 27, 29, 31, 33, and 35 after parasite inoculation. Values are means ± standard errors of the means (n = 3). *** (P < 0.001) indicates the significant difference when comparing the number of parasites per footpad for DSHemsPC-AmB-Lip or AmBisome to empty liposome and 5% dextrose (B and C).