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. 2017 Jul 18;38(4):180–190. doi: 10.24272/j.issn.2095-8137.2017.045

Figure 4.

A: Diagram of two shRNAs targeting tree shrew <italic>p53</italic>; B: Lentivirus expressing shRNAs co-expressing tdTomato infected SKPs <italic>in vitro</italic> efficiently; C: shp53 knockdown efficiency was verified by real-time PCR, cells were SKPs infected with indicated viruses; D: The tree shrew tail skins formed BCC tumors efficiently. Trypan blue, tdTomato, and GFP indicate the locations of the injected viruses; E: Representative images of BCC originated from the tails of tree shrews with indicated treatment (empty vector, SmoA1 or/in combination with shp53, respectively); F: Percentage curve of tumor-free tree shrews shows that the combination of lentiviral SmoA1 and shp53 (median time: two weeks) accelerated BCC formation in tails compared with SmoA1 alone (median time: six weeks). <italic>n</italic>=6, separately.

BCC formation in tree shrew tail skins by combined usage of SmoA1 and shp53 viruses