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. 2017 Mar 11;21(9):2068–2076. doi: 10.1111/jcmm.13127

Figure 1.

Figure 1

The HR/SS cell treatment protocols. Two protocols of hypoxia–reoxygenation (HR) and serum starvation (SS) were employed for the treatment of cells. Scheme A, after seeding, the cells were cultured in starvation medium (containing 0.1% FBS) under hypoxia (1% O2) for 24 hrs followed by treatment with HP for 24 hrs under normoxia (21% O2). Scheme B, after seeding, the cells were cultured in starvation medium (containing 0.1% FBS) under hypoxia (1% O2) for 4 hrs followed by treatment with HP for 30 min. under normoxia (21% O2). Scheme A and Scheme B are used for cell apoptosis and signalling studies, respectively.