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Human plasma induces phosphorylation of BAD and increases its interaction with 14‐3‐3 in endothelial cells. ECs were treated for 30 min. with HP at the indicated concentrations according to the procedure described in Scheme B and subjected to whole‐cell lysate preparation followed by immunoblotting or immunoprecipitation–immunoblotting. IP: immunoprecipitation; IB: immunoblotting; WCL: whole‐cell lysate.
