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. 2017 Mar 11;21(9):2068–2076. doi: 10.1111/jcmm.13127

Figure 4.

Figure 4

Akt is partially responsible for the anti‐apoptotic effect of human plasma. ECs were treated according to Scheme A (4C and 4D) or Scheme B (4A and 4B). (A) ECs treated with different concentrations of HP were subjected to whole‐cell lysate preparation and immunoblotting. (BD), HPMECs were transfected with Akt1 siRNA or a scramble non‐targeting control siRNA using Lipofectamine 2000. Seventy‐two hours after transfection, the cells were starved and treated for 30 min. with or without HP (30%) followed by immunoblotting (B), or subjected to DNA fragmentation (C), or caspase 3/7 activity assay (D). Note: Different lower‐case letters denote significant statistical difference (P < 0.05) among different groups. IB: immunoblotting; RLUs: relative light units.