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PAI1 is responsible for part of human plasma‐mediated BAD phosphorylation and apoptosis protection. ECs were transfected with PAI1 siRNA using Lipofectamine 2000 and subjected to immunoprecipitation–immunoblotting (A), DNA fragmentation (B) or caspase 3/7 activity assay (C). Note: Different lower‐case letters denote significant statistical difference (P < 0.05) among different groups. IB, immunoblotting; WCL, whole‐cell lysate; RLUs, relative light units.
