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. 2017 Apr 4;21(9):1915–1928. doi: 10.1111/jcmm.13113

Figure 4.

Figure 4

Temporal and spatial evolution of myocardial MMP‐9 and MMP‐2 activation and expression after MI. (A) Representative zymography analysis results showing myocardial MMP‐9 and MMP‐2 activity. Bar graphs showing the mean ± S.D. of MMP‐9 and MMP‐2 activity in the remote, peri‐infarct and infarct zones at 1 (B and E), 10 (C and F) and 21 (D and G) days after MI. (H–M) Real‐time PCR showing MMP‐9 and MMP‐2 mRNA expression levels. Data were processed using a specially designed software program based on the Ct values of each sample and normalized to 18s rRNA, which served as an endogenous control. The results are shown as the mean ± S.D. of MMP‐9 and MMP‐2 mRNA expression levels in the remote, peri‐infarct and infarct zones at 1 (H and K), 10 (I and L) and 21 (J and M) days after MI. n = 8. *P < 0.05 versus remote; **P < 0.01 versus remote; ## P < 0.01 versus peri‐infarct; ***P < 0.005 versus remote; ### P < 0.005 versus peri‐infarct.