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. 2017 Apr 4;21(9):1915–1928. doi: 10.1111/jcmm.13113

Figure 8.

Figure 8

Comparison of the hypoxia effect on pPyk2 levels and MMP‐9/MMP‐2 activation in control (Lrp1 +/+) and LRP1‐deficient fibroblasts (Lrp1 −/−). Quiescent Lrp1 +/+ and Lrp1 −/− fibroblasts were exposed to normoxic or hypoxic conditions for 18 hrs. (A) Representative Western blot analysis showing LRP1, pPyk2, total Pyk2 and β‐tubulin levels and bar graphs showing the mean ± S.D. of LRP1 levels normalized to β‐tubulin (B) and pPyk2/total Pyk2 ratio (C). (D) Representative zymography analysis showing MMP‐9 and MMP‐2 activity levels and bar graphs showing the mean ± S.D. of MMP‐9 (E) and MMP‐2 activity levels (F). Results are shown as the mean ± S.E.M. of three independent experiments performed in triplicate. ***P < 0.001 versus normoxia. (G) Representative scheme showing the crucial role of LRP1 up‐regulation in hypoxic cardiac fibroblasts in cardiac remodelling.