Figure 4.

I/R induced GSK‐3β activation via PKC‐δ‐dependent pathway in ALDH2^−/− mice. (A) The cellular expression of GSK‐3β and p‐GSK‐3β^{Ser 9} in ALDH2^−/− mice and WT littermates (B) with the pre‐treatment of NAC 1 hr before ischaemia. (C) Western blot analysis of GSK‐3β and p‐GSK‐3β^{Ser 9} in ALDH2^−/− mice pre‐treated with PKC‐δ siRNA and scrambled siRNA as control. (D) GSK‐3β expression at the mitochondrial fractions in ALDH2^−/− hearts at indicated time, I_{45 min.}/R_{60 min.} and I_{45 min.}/R_{24 hrs}; GSK‐3β translocation at I_{45 min.}/R_{24 hrs} in comparison with the pre‐treatment of NAC, PKC‐δ siRNA and scrambled siRNA. *P < 0.05, vs C57BL/6 mice