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. 2017 Mar 21;21(9):1767–1780. doi: 10.1111/jcmm.13098

Figure 6.

Figure 6

S256 phosphorylation of AQP2 in V1a receptor knockout mice. Fresh kidney slices were left untreated or stimulated as described in concise methods. Equal amount of proteins (15 μg) were immunoblotted with antibodies specific for total AQP2 or for AQP2‐pS256. Densitometric analysis (right panel) and statistical studies (means ± S.E.Ms) revealed that tolvaptan significantly prevented the increase in AQP2‐pS256 elicited by dDAVP (**P < 0.001 versus CTR; *P < 0.01 versus CTR; #P < 0.01 dDAVP versus dDAVP+TLV; $P < 0.01 FK versus FK+TLV; n = 3).