Figure 2.

P14 immunization on WKY rats induced severe crescentic glomerulonephritis. Immunofluorescent staining of IgG was detectable along GBM in positive controls (A) and P14 immunization rats (D), but not in negative controls (G). Crescent formation was observed in positive controls (B) and P14 immunization rats (E), but not in negative controls (H). On electron microscope, the fracture and shrinking of GBM was identified in the glomeruli with crescent formation in positive controls (C) and P14 immunization group (F), without electron dense deposits. No GBM damage was observed on electron microscope in negative controls (I). BC: Bowman's capsule.