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. 2017 Mar 10;21(9):2117–2128. doi: 10.1111/jcmm.13134

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© 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Immune responses to the P14 immunization on WKY rats. Serum antibodies from P14‐immunized rats recognized the immunogen peptide P14 and human α3(IV)NC1 protein (A). The binding between anti‐P14 antibodies and P14 was inhibited by P14 itself but not by α3(IV)NC1, using antigen inhibition ELISA (B). The elutes from P14‐immunization rat kidneys recognized P14, human α3(IV)NC1 and α2(IV)NC1 (C). Antigen‐specific lymphocyte proliferations towards P14 and α3(IV)NC1 were detectable in P14‐immunization rats, but not in negative controls (D). Cut‐off line (…) showed the level taken as representing positive response (SI = 2.0). *P = 0.014 compared with negative control.