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Serum antibodies from the rats immunized with sequential amino acid substituted peptides. Serum antibody recognized the immunogen itself but not intact α3(IV)NC1 in the rats immunized with tryptophan136, isoleucine137, leucine139 or tryptophan140 substitution peptide of P14 (A–D). No antibody was found towards the immunogen or α3(IV)NC1 in the rats immunized with phenylalanine143 substitution peptide of P14 (E).
