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. 2017 Apr 4;21(9):2153–2162. doi: 10.1111/jcmm.13138

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© 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Chondrogenesis of stem cells from SC adipose tissue and VS adipose tissue in micromass cultures. (A and B) S‐ASCs in micromass cultures were treated with chondrogenic media at different times. Cultures were stained with Alcian blue (A) and Western blot analysis of the lysates (40 μg) (B) (C–F) the chondrogenesis capabilities of S‐ASCs and V‐ASCs were evaluated by Alcian blue staining (C); diameter of the micromass; (D) (n = 4), Western blot analysis of lysates (40 μg); (E) and relative mRNA expression of the indicated chondrocyte‐related markers (F) (n = 3) with non‐differentiated or differentiated medium on day 21. *P < 0.05, **P < 0.01, ***P < 0.001.