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. 2017 Mar 22;21(9):2172–2183. doi: 10.1111/jcmm.13141

Figure 2.

Figure 2

The relevance between the regulation of STAT3 and STAT5 activities and the inhibition of Th17 cell differentiation by the tetrandrine treatment during the Th17 cell differentiation. The effect of tetrandrine on the Treg cell differentiation during the Th17 cell differentiation was investigated. The naïve CD4+ T cells were stimulated with anti‐CD3/CD28 and were treated with TGF‐β and IL‐6 in the presence or absence of tetrandrine (TET) and HPA (an inhibitor of STAT5) for 72 hrs. (A and B) The frequency of Treg cells in the CD4+ T cells was determined using the flow cytometry assay. (C) The expression of the mRNAs for the transcription factor Foxp3 and the cytokine IL‐10 was determined using the quantitative PCR assay. The colivelin (CON, an activator of STAT3) or the HPA was added into the naïve CD4+ T cells together with tetrandrine for 72 hrs. (D and E) The frequency of Th17 cells in the CD4+ T cells was determined using the flow cytometry assay. (F) The expression of the mRNAs for the transcription factor RORγt and the cytokine IL‐17A was determined using the quantitative PCR assay. The data are from the three independent experiments. **P < 0.01 versus the vehicle‐treated group; ## P < 0.01 versus the tetrandrine‐treated group.