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. 2017 Feb 23;21(9):1781–1790. doi: 10.1111/jcmm.13099

Figure 4.

Figure 4

Inhibition of ERK2 has no effect on TGF‐β1‐induced TIMP‐3 expression. (A and B) Real‐time PCR and Western blotting indicated ERK2 knock‐down had no effect on TGF‐β1‐induced TIMP‐3 expression (NS = no significant difference). (C and D) At 0‐, 10‐, 15‐, 30‐ and 60‐min. time‐points, Western blotting revealed that p‐ERK2 was inhibited by LV expressing ERK2 siRNA2 following TGF‐β1 stimulation. The intensity levels of p‐Smad3, p‐ERK1 and p‐ERK2 were quantified. Data shown are from one representative experiment out of the three performed.