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. 2017 Mar 27;21(9):2211–2222. doi: 10.1111/jcmm.13144

Figure 3.

Figure 3

Effect of cigarette smoke exposure and let‐7f treatment on ischaemia‐induced neovascularization. A and C. Representative images (A) and quantification (C) of laser Doppler measurements after hindlimb ischaemia in control mice (CTL) and in MES treated with let‐7f or a scrambled miR control (mimic C). A colour scale illustrates blood flow variations from minimal (dark blue) to maximal (red) values. Arrows indicate region of interest in left ischaemic hindlimbs (n = 10–12/group). B and D. Representative images (B) and quantification (D) of CD31 immunostaining in ischaemic (isch) and non‐ischaemic (n‐isch) hindlimb muscles of the different groups of mice (n = 12–13/group). E–F. Percentage of mice walking normally (E) and presenting no ischaemic damage (F) in the different groups at day 21 after ischaemia (n = 11–15/group). *P < 0.05 versus CTL+mimic C; #P < 0.05 versus MES+mimic C.