Table 1.
Top 30 significantly DE miRNAs and their predicted target genes from microarray analysis in PMECs at 3 hpc with E. coli compared with unchallenged controls
| 3 hpc with E. coli | ||
|---|---|---|
| Upregulated miRNA | Sequence (5′ - > 3′) | Downregulated target genes |
| miR-29b | UAGCACCAUUUGAAAUCAGUGUU | BCOR, KLHDC7A |
| miR-140 | CAGUGGUUUUACCCUAUGGUAG | RAB40B |
| miR-210 | UUGUGCGUGCGACAGCGACUUC | RAB40B |
| miR-101 | UACAGUACUGUGAUAACUGAAG | EML5, HELLS, RBM12 |
| miR-29a | UAGCACCAUUUGAAAUCGGUU | CDC6, CYP26A1, DNAJA1, EML5, RNF2, TIMM44 |
| miR-24-star | GUGCCUACUGAGCUGAUAUCAGU | USP46 |
| miR-30b-star | CUGGGAGGUGGAUGUUUACUUC | BCOR |
| miR-B6 | AAGUGCCCGACGCGGGGAACGUG | ENC1 |
| let-7 g-star | CUGUACAGGCCACUGCCUUGC | DUSP6 |
| miR-125a-3p | ACAGGUGAGGUUCUUGGGAGCC | ARID5B, ID4, ITGB8, KIAA0182, NUAK1, PHF17, RAB40B, TOX, USP2, VGLL3, WDR35 |
| Downregulated miRNA | Sequence (5′ - > 3′) | Upregulated target genes |
| miR-371 | ACUCAAAAAAUGGCGGCACUUU | SGSM1 |
| miR-548u | CAAAGACUGCAAUUACUUUUGCG | NFKBIA |
| miR-5128 | CAAUUGGGGCUGGCGAGAUGGCU | SGSM1 |
| miR-8-5p | CAUCUUACCGGGCAGCAUUAGA | ARHGAP28, HIVEP2, IFNAR2 |
| miR-259-star | CCACCGAUUUGGCAUGGGAUUGAC | SGSM1 |
| miR-3277 | UGGGCAUGUUUCUGAAAUUCGAU | ARHGAP28, IFNAR2, IL1A, PPARG |
| miR-4903 | UACCCCGGUAGGCAUAGGUUG | GLRX, IL1RAP, MAP3K8, PLAUR |
| miR-202 | AGAGGCAUAGGGCAUGGGAAAA | ARHGAP28, CHL1, GLI3, HIVEP2, IFNAR2, JAG1, LIFR, MAP3K8, OSBPL3, P2RX4, RFFL, RMND5A, RNF19B, TEP1, TNFAIP3, TPM2, VPS37C, YPEL2 |
| miR-5100 | UCGAAUCCCAGCGGUGCCUCU | OBFC1 |
| miR-2287 | CUGGGACUGGUGGCAGCACUU | CLDN1, ITGAV, RNF19B |
| miR3949 | UGAUGUUGAGGCAAAAAUGUAG | CCDC152, RBM39, RFX1 |
| miR-885-3p | AGGCAGCGGGGUGUAGUGGAUA | BIRC3, DCUN1D3, HIVEP2, IL10RB, MAP3K8, TNFAIP3 |
| miR-2518 | UCGAGCAGCGGGUCGAUCCGAGC | CYP1A1, JAG1 |
| miR-3042 | GAGGGCAGAUUAUUUCUGAUAC | JAG1, LPIN1, YPEL2 |
| miR-4031-5p | CCCAAAGUGUCGGCGCAUAU | SGSM1, TNFAIP3 |
| miR-4317 | ACAUUGCCAGGGAGUUU | NFKBIA |
| miR-216b | AAAUCUCUGCAGGCAAAUGUGA | ETV5, PDLIM5 |
| miR-890 | UACUUGGAAAGGCAUCAGUUG | EHF, FCHSD2, IL10RB, PLXND1, SGSM1, SLC4A7, TAP1 |
| miR394b-star | AGGUGGACAUAUUGCCAACA | ITGAV, JAG1 |
| miR-648 | AAGUGUGCAGGGCACUGAU | HIVEP2, IFNAR2, JAG1, SGSM1 |
MiRNAs with p < 0.05 and fold change >1.5 or < −1.5 are listed and sorted by fold change in descending order. TargetScan-predicted miRNA-mRNA pairs showing negative correlation in expression profile and energy-value of at least −25 kcal/mol