Table 2.
Top 30 significantly DE miRNAs and their predicted target genes from microarray analysis in PMECs at 24 hpc with E. coli compared with unchallenged controls
| 24 hpc with E. coli | ||
|---|---|---|
| Upregulated miRNA | Sequence (5′ - > 3′) | Downregulated target genes |
| miR-505 | CGUCAACACUUGCUGGUUUCCU | ABCA7, DDX18, DDX52, EIF4E, GRPEL1, LARP4, MSL1, PLEKHH1, SDC1, SERTAD1, SOCS4, TATDN2, WDR3, YTHDF2, ZBED4 |
| miR-29a | UAGCACCAUUUGAAAUCGGUU | CDC6, CYP26A1, DNAJA1, EML5, RNF2, TIMM44 |
| miR-101 | UACAGUACUGUGAUAACUGAAG | CHD7, EML5, HELLS, LARS, MID1, RBM12 |
| miR-24-star | GUGCCUACUGAGCUGAUAUCAGU | USP46 |
| miR-289 | UAAAUAUUUAAGUGGAGCCUGCGACU | KIAA1430, RNF2, TMCC3 |
| miR-125-star | ACGGGUUAGGUUCUUGGGAGC | GIT2, LARS, MSL1, PAFAH1B1, RBM12, SDC1, TMEM79, UBE2R2, ULK3, USP46 |
| miR-484 | UCAGGCUCAGUCCCCUCCCGAU | FGFR3 |
| miR-22-star | AGUUCUUCAGUGGCAAGCUUUA | ARGLU1 |
| miR-660 | UACCCAUUGCAUAUCGGAGCUG | ARMC8, BCLAF1, CAMKK2, CPT1A, DDX42, MSL1, NOL6, NRARP, PLEKHH1, PPP1R8, PPP6C, PSMD12, PSMF1, PYGO2, RRP12, SDC1, TRIM27, ZFX, ZNF280D |
| miR-B6 | AAGUGCCCGACGCGGGGAACGUG | ENC1 |
| miR-363 | AAUUGCACGGUAUCCAUCUGUA | ABCA7, ADPRH, ATXN1, CAMKK2, DGCR8, FAM53C, GABPB1, GATAD2A, HERPUD2, PAFAH1B1, PLEKHH1, POP4, PPRC1, RBM15, S1PR2, SYK, TATDN2, TFAP2C, TRIM35, TXLNA |
| miR-128 | UCACAGUGAACCGGUCUCUUUU | ADPRH, AQP3, GABPB1, KCNJ14, NDNL2, PLEKHH1, RRP12, SDC1, SNX6, TCEB3, UBE2R2, ULK3, ZFAND2A, ZFX, ZNF764 |
| miR-10a | UACCCUGUAGAUCCGAAUUUGUG | ADPRHL2, EML5, FARSA, FEN1, IQCC, KLHL18, LMNB2, MFSD9, SFN, SLC16A3, SLC39A6, TATDN2, TLE1, TMCC3, TUBB2B, TXLNA, UBAP2L, ZNF367 |
| miR-92b | AAUUGCACUAGUCCCGGCCUGC | CPT1A, GPRC5A, HEXIM1, LIN37, PHF23, PKP1, ULK3, YWHAG |
| miR-92b | UAUUGCACUCGUCCCGGCCUCC | EML5 |
| Downregulated miRNA | Sequence (5′ - > 3′) | Upregulated target genes |
| miR-371 | ACUCAAAAAAUGGCGGCACUUU | SGSM1 |
| miR-4423-3p | AUAGGCACCAAAAAGCAACAA | RASAL2 |
| miR5019 | UGUUGGGAAAGAAAAACUCUU | RASAL2 |
| miR-3128 | UCUGGCAAGUAAAAAACUCUCAU | SLC2A12 |
| miR5021 | UGAGAAGAAGAAGAAGAAAA | CIRBP |
| miR-M9-3p | AAACUCCGAGGGCAGGAAAAAG | ARHGAP12, ARHGAP28, BIRC3, CEP68, FCHSD2, GRK5, ISG15, LPIN1, MDM4, PCDH7, PDLIM5, SLC2A12, TCTN2, YPEL2 |
| miR3949 | UGAUGUUGAGGCAAAAAUGUAG | CCDC152, RBM39, RFX1, |
| miR-B8-5p | CGCGGGCAAAAAAUCCAAUGGC | BIRC3, MAP3K8, PLXND1, RNF19B |
| miR-2326 | CCCCCCUUCCUCUGGAAAAA | ARHGAP28, CEP68, CHI3L1, CXCL2, FNBP1, LPIN1, PCDH7, PLXND1, PPAP2B, RFFL, RHOQ, SCUBE3, SLC4A7, TPM2 |
| miR-5100 | UCGAAUCCCAGCGGUGCCUCU | OBFC1 |
| miR-259-star | CCACCGAUUUGGCAUGGGAUUGAC | SGSM1 |
| miR-2779 | AUAUCCGGCUCGAAGGACCA | CLDN1, PLXND1 |
| miR-4592 | CCAGCGGCGGUGCCGUGAUGGCGA | CLDN1, CEP68, CXCL2, ITGB8, MDM4, RHOQ |
| miR-202 | AGAGGCAUAGAGCAUGGGAAAA | ARHGAP28, BIRC3, CHL1, GLI3, LIFR, MAP3K8, P2RX4, PLN, RASAL2, RFFL, RMND5A, RNF19B, SGSM1, TEP1, TNFAIP3, TPM2, VPS37C |
| miR-3277 | UGGGCAUGUUUCUGAAAUUCGAU | ARHGAP28, IFNAR2, IL1A, PPARG |
MiRNAs with p < 0.05 and fold change >1.5 or < −1.5 are listed and sorted by fold change in descending order. TargetScan-predicted miRNA-mRNA pairs showing negative correlation in expression profile and energy-value of at least −25 kcal/mol