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Solubilization of microvillus vesicles by LC, DG, and C12E9. Microvillus membranes were prepared as described in Methods and treated with PEs at a range of concentrations as indicated. After treatment, the pellet (P) -and supernatant (S) fractions following a centrifugation were subjected to SDS/PAGE. Arrows indicate the molecular mass-values of the brush border enzymes sucrose-isomaltase (250 kDa), aminopeptidase N (150 kDa) and the cytoskeleton protein actin (42 kDa).
