Table 1. Bacterial strains and plasmids used in this study.
Strain/plasmid |
Genotype/relevant characteristics |
Reference |
Escherichia coli AG115 | lacX74, galU, galK, araD139, strA, hsdR17/F’, lacIq lacZ::Tn10 | (39) |
Escherichia coli DH5α | supE44, hsdR17, recA1, endA1, gyrA96, thi-1, relA1 | (11) |
H.pylori J99 | cagA+ vacA+ isolated from a patient with duodenal ulcer | (7) |
pGEX-KG | Ptac, gst, oripBR322 | (40) |
GHPAQ41 | ATCCa Molecular Biology, pUC18 derivative containing the entire H.pylori dnaA gene (1741 bp fragment) | (6) |
pGEXHpdnaA(IV) | pGEX-KG derivative containing the 343 bp EcoRI–BamHI fragment encoding domain IV of H.pylori DnaA protein | This study |
pOC170 | pBR322 derivative containing the E.coli oriC region (five DnaA boxes) | (41) |
aATCC, American Type Culture Collection.