Fig 1. Generation of Trp53/Pten mice associated with luciferase expression.

A) ROSA26 Lox-stop-Lox Luciferase, Pten^fl/fl male mice were crossed with Trp53^fl/fl female mice to generate offspring with ROSA26 LSL-Luc, Pten^fl/fl, and Trp53^fl/fl. Ad with a Cre recombinase expression vector was injected either subcutaneously over the right leg/flank or intramuscularly into the right quadriceps to promote somatic recombination. Somatic expression of Cre recombinase promoted expression of luciferase and homozygous knockout of Pten and Trp53. B) PCR from DNA isolated from either a tumor of Cre injected mice (Cre+) or from the tail of a non-injected mouse (Cre-) (Ctrl is a primer only control) to assay for the presence of recombination and floxed Pten or Trp53.