Fig 2. Trp53/Pten mice injected with Ad CMV-Cre demonstrate a longitudinal increase in BLI signal.

A) BLI of Trp53/Pten mice injected either SQ or IM with Ad CMV-Cre. Mice were injected with intraperitoneal D-luciferin and imaged using an Ami X imager. Shown are representative images of five mice from each cohort at 1 week, 9 weeks, and 17 weeks post-viral injection, demonstrating a steady increase in luciferase signal over time. B) Quantified whole-body in vivo BLI values as measured in photons/sec/cm²/sr are shown for individual mice for each injection group (n = 9), and compared between the two injection groups C), highlighting similar luciferase expression kinetics and values between SQ- and IM-injected animals. The difference between SQ and IM injection was statistically significant for day 1 post-injection (p = 0.045), but not thereafter. From the 2way ANOVA, overall variance due to injection method was statistically significant (p = 0.0019). In comparison to the first day post-injection, all values for both injection methods were significant (p<0.0001).