Fig 3. IM-injected Ad CMV-Cre results in increased systemic viral spread.
A) Quantified leg-only or abdomen-only BLI values are shown for individual Trp53fl/flPtenfl/fl mice injected either SQ or IM with Ad CMV-Cre. B) Comparisons between injection cohorts demonstrate lower non-specific signal in the abdomen with SQ injection. There was no significant difference in luciferase signal between the SQ- or IM-injected legs; however, there is a significant difference between SQ and IM abdomen injections for week 1 and beyond (p<0.05). The difference in comparison to the first day post-injection was statistically significant for both SQ (p<0.002) and IM (p<0.0001) leg injections as well as IM abdominal BLI (p<0.02). No significant difference was found for SQ abdominal BLI in comparison to day 1. C) Ex vivo BLI images of livers from one SQ- and one IM-injected mouse, depicting multifocal low-level recombination and luciferase expression in the livers of mice with abdominal BLI signal, suggesting systemic viral spread and infection (left panels). Histological sections of liver demonstrated extramedullary hematopoiesis, noted with arrows, but no neoplasm was identified (right panel).