Fig 3. Detection of HI antibody titer and serum IgG, IgG1, IgG2a of antigen sparing.
(A) HI antibody titer against different doses of V antigen after vaccination of V 0.05 μg, V 0.1μg, and V 0.5 μg mixed with 300 μg of AEAR on day 21 after first vaccination. (B) Detection of IgG titer in serum by indirect ELISA on day 7 after first immunization. (C) IgG titer in serum on day 7 and 14 after first immunization. (D) Detection of antibody IgG, IgG1, IgG2a in serum on day 14 and 21 after first immunization. Data were expressed as means SE (n = 6). The results were representatives of three independent experiments. * P<0.05, ** P<0.01, ns P>0.05.