Fig 6. Analysis of antigen-specific cytokine production in T cells by FACS.

T cells isolated from the spleen of ICR mice on day 7 after second immunization. The percentages of IL-4 (A-B) in CD4⁺, IFN-γ(C-D) in CD4⁺, IFN-γ(E-F) in CD8⁺ were shown. Data were expressed as means±SE (n = 6). The results were representatives of three independent experiments. * P<0.05, ** P<0.01, ^ns P>0.05.