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. 2017 Aug 25;12(8):e0183680. doi: 10.1371/journal.pone.0183680

Fig 1. Tunicamycin(TM) induces ER stress, which in turn results in apoptosis of HCC cells.

Fig 1

(A) Hepa 1–6 cells were treated with 0.8 μg/mL of TM for 0, 8, 12, or 24 h. Cells were collected and subjected to total RNA extraction. The mRNA levels of ER stressrelated genes Grp94 and Grp78 were measured by quantitative RT-PCR. All results were normalized with the internal control β-actin. Bars, SD. Data represent3 independent experiments.(B) Hepa 1–6 cells underwent aforementioned treatment were collected and subjected to western blot analyses with specific antibodies directed against Grp78, Grp94, p-eIF2α, eIF2α, c-PARP, or β-actin. The density of the corresponding bands was measured quantitatively using image analysis software and corrected by reference to the value of β-actin. Each data point is the mean ± SD of 3 independent experiments, *P<0.05 or **P<0.01 denotes significant difference from normal control Hepa1-6 cells.