Fig 1. ROCK1 protein expression and gene amplification in PDAC tissues.

A) Immunostaining of ROCK1 protein in a normal pancreas section (negative staining) and a PDAC tissue section (strong staining with a score of 3+). Black arrow: tumor cells; oval arrow: cancer associated fibroblasts. B) aCGH plots of the whole genome (bottom panel), chromosome 18 (middle panel) and the ROCK1 gene locus (top panel) from a representative patient with a focal (1.76Mb) 18q11.1 amplicon. Blue shaded areas denote aberrant copy number intervals defined by ADM2 step gram algorithm [31] with a log₂ratio of +1.4 (p<0.001) for the ROCK1 containing amplicon.