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Influence of NPM1 knockdown on plasmid DNA transfection.
| qPCR | Assessment | Location | Samples | DNA Copies/108cells | % Input | Ratio |
|---|---|---|---|---|---|---|
| Target | N/S | |||||
| input | 6.8x x1013 | 100% | ||||
| DNA Transfection | Whole cells | Scramble | 5.51±0.43 x1012 | 8.10% | ||
| Rep | NPM | 6.49±0.19 x1012 | 9.54% | 1.18 | ||
| Cytoplasm | Scramble | 2.73±0.57 x1012 | ||||
| NPM | 1.57±0.76 x1012 | 0.57 | ||||
| Nuclei | Scramble | 4.49±0.38 x1011 | ||||
| NPM | 1.01±0.25 x1012 | 2.25 |
