Table 1. List of the primers required for the ADDER cDNA display.
|
Upstream primers (5′→3′) |
|
Downstream primers (5′→3′) |
1 |
CCAACGGATCGGa |
17 |
AGCTTTTTTTTTTTTGG |
2 |
AACCGATCGA |
18 |
AGCTTTTTTTTTTTTAA |
3 |
AACCGATCGT |
19 |
AGCTTTTTTTTTTTTCT |
4 |
AACCGATCGC |
20 |
AGCTTTTTTTTTTTTGC |
5 |
AACCGATCAG |
21 |
AGCTTTTTTTTTTTTAG |
6 |
AACCGATCAA |
22 |
AGCTTTTTTTTTTTTCA |
7 |
AACCGATCAT |
23 |
AGCTTTTTTTTTTTTGT |
8 |
AACCGATCAC |
24 |
AGCTTTTTTTTTTTTAC |
9 |
AACCGATCTG |
25 |
AGCTTTTTTTTTTTTCG |
10 |
AACCGATCTA |
26 |
AGCTTTTTTTTTTTTGA |
11 |
AACCGATCTT |
27 |
AGCTTTTTTTTTTTTAT |
12 |
AACCGATCTC |
28 |
AGCTTTTTTTTTTTTCC |
13 |
CCAACCGATCCG |
|
|
14 |
CCAACCGATCCA |
|
|
15 |
AACCGATCCT |
|
|
16 | AACCGATCCC |
aThis primer was modified to prevent self-annealing, as described in Materials and Methods.