Figure 1.

Screening potent inflammation inhibitor in natural compounds from Premna szemaoensis Pei extracts. (A) Mouse peritoneal macrophages were pretreated with DMSO or various compounds (25 μM) for 2 h, following stimulated with LPS (300 ng/ml) for 3 h and then treated with nigericin (10 μM) for 45 min. ELISA was used to analyze IL-1β secretion. (B) The chemical structure of Teuvincenone F. (C) The X-ray of Teuvincenone F. (D,E) Mouse peritoneal macrophages (D) and THP1 cells (E) were cultured with different concentrations of Teuvincenone F (0, 6.25, 12.25, 25 μM) for 12 h, the cell viability was determined by MTT assay. Data are shown as mean ± SD of one representative experiment in (A,D–E). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.