Table 1.

Identification of proteins #1–35 by Orbitrap Velos or Q Exactive mass-spectrometer.

Spot	Protein	Accession No	Symbol	MWa)	p1b)	Peptidesc)	% coveraged)
1	Collagen 1 alpha-2 chain	NP_031769	Col1A2	129.6	9.3	7	5.9
2	Elongation factor 2	NP_031933	Eef2	95.3	6.4	14	14.0
3	Moesin	NP_034963	Msn	66.5	5.9	51	70.9
	Caldesmon-1	NP_663550	Cald1	60.5	7.0	46	69.2
4	Dihydropyrimidinase-like 3	NP_001129558	Dpysl3	61.9	6.0	12	26.4
5	Serine protease HTRA1	NP_062510	Htra1	51.2	7.8	22	45.0
6	Serpin H1	NP_001104514	Serpinh1	46.5	8.9	13	30.0
7	Serine-threonine kinase receptor-associated protein	NP_035629	Strap	38.4	5.0	4	16.9
8	Tropomyosin 3	NP_071709	Tpm3	32.8	4.2	26	60.2
9	14-3-3 protein epsilon	NP_033562	Ywhae	29.2	4.6	28	74.9
10	Protein-lysine6-oxidase	NP_034858	Lox	46.7	8.7	6	15.8
11	Annexin A2	NP_031611	Anxa2	38.6	7.6	5	32.4
12	Rho dissociation inhibitor 2	NP_031512	Arhgdib	22.9	5.0	13	57.0
13	Carbonic anhydrase 3	NP_031632	Car3	29.4	6.9	6	24.6
14	Peptidyl-prolyl cis-trans isomerase C	NP_032934	Ppic	22.8	7.0	2	11.8
15	Protein S100-A11	NP_058020	S100a11	11.0	5.3	6	51.0
16	Phosphodiesterase 4D, cAMP-specific (Fragment)	NP_035186	Pde4d	84.5	4.8	2	2.3
17	Protein S100-A13	NP_033139	S100a13	17.7	6.2	5	43.9
18	Cytochrome c oxidase subunit 6B1	NP_079904	Cox6b1	10.1	9.0	2	11.6
19	Protein S100-A6	NP_035443	S100a6	10.1	5.3	2	16.9
20	Secernin-1	NP_081544	Scrn1	46.3	4.7	7	15.2
21	Connective tissue growth factor	NP_034347	Ctgf	38.6	7.6	3	12.1
22	Transgelin (SM-22)	NP_035656	Tagln	22.5	8.9	17	63.2
23	Hemoglobin subunit beta-1	NP_032246	Hbb-b1	15.7	7.1	7	49.7
24	Myosin, light polypeptide kinase	NP_647461	Mylk	213.6	5.9	14	15.2
	Protein phosphatase 1 regulatory subunit 12A	NP_082168	Ppp1r12a	111.8	5.5	6	6.3
25	Myh11 protein	NP_038635	Myh11	227.1	5.4	19	10.4
26	Leucine zipper transcription factor-like 1	NP_201579	Lztfl1	34.7	5.1	6	21.5
27	Sepiapterin reductase	NP_035597	Spr	27.9	5.9	6	37.4
	Heat shock protein beta-1	NP_038588	Hspb1	23.0	6.1	5	31.4
28	Myosin regulatory light polypeptide 9	NP_742116	Myl9	19.9	4.8	6	33.7
29	Cofilin-2	NP_031714	Cfl2	18.7	7.7	9	51.8
30	Cofilin-1	NP_031713	Cfl1	18.6	8.2	8	51.8
31	Prefoldin subunit 2	NP_035200	Pfdn2	16.5	6.2	7	45.5
32	Fatty acid-binding protein, heart	NP_034304	Fabp3	14.8	6.1	8	54.9
33	Destrin	NP_062745	Dstn	18.5	8.1	8	49.1
34	Glutathione S-transferase Mu	NP_034488	Gstm1	26.0	7.7	11	47.2
35	Mimecan (Osteoglycin)	NP_032786	Ogn	34.0	5.5	5	13.8

CPFP version 2.0.3 was used for database searching against the Uniprot mouse database.

^a)Theoretical molecular weight (KDa).

^b)Theoretical isoelectric point (pI).

^c)Number of matched peptides.

^d)Percentage of sequence coverage.