Figure 2.

PKC activation cause a dynamin dependent reduction in surface expression of NET. PC12 cells stably transfected with an HA-tagged NET were treated with PKC activator PMA and dynamin inhibitor Dynole 34-2. Cell surface biotinylation was performed and surface expressed NET was visualized through immunoblotting (A). Band densities were quantified from three independent experiments (B). Statistical significance was determined employing one-way ANOVA with Bonferroni post hoc analysis (**P < 0.01). P:PMA (1 μM), D:Dynole 34-2 (20 μM).