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. 2017 Aug 22;11:253. doi: 10.3389/fncel.2017.00253

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Copyright © 2017 Mortensen, Larsen and Amara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PKC-mediated decrease in NET surface expression is attenuated by MAP kinase phosphatase 3 (MKP3). Cell surface biotinylation was performed on naïve (PC12) and MKP3-expressing (MKP3) PC12 cells following PKC activation by PMA (1 μM) for 30 min. NET surface expression was visualized by immunoblotting (A). Band densities were quantified from three independent experiments (B). Statistical significance was determined employing two-way ANOVA with Bonferroni post hoc analysis (*p < 0.05 comparing surface expression following PMA treatment).