Figure 2.

Dyslipidemic diet (DD)-induced metabolic priming of baboon monocytes is correlated with hypercholesterolemia. (A) MCP-1-dependent chemotaxis of purified baboon monocytes that were cultured overnight in complete RPMI culture media with 5 mM glucose (C) or media containing an additional 20 mM glucose and 100 µg/mL low-density lipoprotein (LDL) (HG + LDL). Chemotaxis data (mean ± SEM) were normalized to the control group at each time point and is expressed as “Fold Induction.” (B) Cell viability of purified baboon monocytes described in panel A (mean ± SEM). NS, not statistically significant. (C) MCP-1-dependent chemotaxis of purified monocytes from baboons that were fed an maintenance diet (MD) (□) or a DD (■) for either 4 or 8 weeks (mean ± SEM). (D) Linear regression analysis assessing the relationship between serum cholesterol levels and monocyte chemotaxis in baboons fed an MD for 4 weeks (□) or 8 weeks (⚪), or a DD for 4 weeks (■) or 8 weeks (⚫). Exact P-values are shown for the chemotaxis data (A,C), and the exact r² and P-values for the correlation analysis are shown (D).