Table 1.

IC₅₀ (μg/mL) assayed for doxorubicin resistance in 2D and 3D cultures.

	Cell-seeding concentrations (×104 cells/mL)
	0.5		1.25		2.5		3.75
Culture model	2D	3D ULA	2D	3D ULA	2D	3D HD	2D	3D HD
IC50 (μg/mL)	0.37	1.0**##	0.43	0.99**##	0.37	0.55*	0.4	0.83**#
Confidence Interval 95%a	0.32–0.44	0.84–1.2	0.34–0.54	0.83–1.18	0.28–0.49	0.44–0.68	0.30–0.52	0.66–1.04

Extra-sum-of-squares statistic method with F test:

^*P < 0.05 vs. 2D cultures with the same cell seeding;

^**P < 0.001 vs. 2D cultures with the same cell seeding;

^#P < 0.05 vs. 3D HD with cell seeding of 2.5 × 10⁴cells/mL;

^##P < 0.001 vs. 3D HD with cell seeding of 2.5 × 10⁴cells/mL;

^aConfidence interval (95%) related to the comparative analysis between 3D and 2D cultures with the same cell seeding.