Figure 2.

Wip1 intrinsically regulates neutrophil migration into the infection sites to improve the outcome of sepsis. Complete chimeras were generated by transferring 1–2 × 10⁷ bone marrow cells from either wild-type (WT) or Wip1 KO mice into lethally irradiated CD45.1 syngeneic mice. (A) Different complete chimeras were established and significantly improved survival rate was found in mice with a Wip1 deficiency in immune cells (P < 0.05, n = 20). Peritoneal exudates were harvested and analyzed for neutrophil accumulation (CD11b⁺Ly6G⁺) by flow cytometry at 3 and 6 h after cecal ligation and puncture (CLP) procedure in WT or Wip1 KO mice, and the representative flow cytometry data of neutrophil percentage (B) were shown between WT and Wip1 KO mice. The number (C) of neutrophils in the peritoneal cavity was shown (mean ± SEM, n = 6–8). (D) WT and Wip1 KO mice received either control IgG (250 µg/mouse) or anti-Ly6G Ab (250 µg/mouse) 24 h before CLP surgery. The survival was monitored for 7 days (n = 10). (E) Neutrophils (3 × 10⁶/mouse) from bone marrow of WT or Wip1 KO mice were adoptively transferred (i.v.) into neutrophil-depleted syngeneic WT mice (3 × 10⁶/mouse) after CLP surgery. All the data are composite of two independent experiments. *P < 0.05, **P < 0.01.