FIGURE 1.

Extracellular P. aeruginosa growth during hCF-infection in the presence of antibiotics. hCF monolayers were infected with different concentrations of P. aeruginosa, ∼10⁵ CFU/ml (MOI = 1), ∼10⁶ CFU/mL (MOI = 10) and ∼10⁷ CFU/mL (MOI = 100), and treated with the different antibiotics (CIP, LVX, PMB, GEN, OFX, CXM and CHL) at 50 and 200 μg/mL. Bacterial growth (CFU) was quantified from n = 3 monolayers at 0, 1, 3, 6, 9, and 24 h. The symbols represent the mean values for CFU/mL of bacterial growth in antibiotic-containing medium from n = 3 independent experiments and the error bars the standard error of the means (SEM). A one-way ANOVA with Dunnett’s multiple comparison test was used to compare the reductions in CFU numbers after antibiotic treatment over time with the initial infecting MOIs (CFU at time = 0 h).