FIGURE 4.

Effect of antibiotic treatments against hCF-intracellular P. aeruginosa. Antibiotic treatment at 50 and 200 μg/mL in hCF monolayers infected with PAO1 wild-type (MOI = 1, 10, and 00). The symbols represent the mean and the error bars the SEM from n = 3 independent experiments, of intracellular PAO1 CFU that was enumerated by viable counting onto agar plates after 1.5, 4.5, 7.5, and 24 h of each antimicrobial treatment that was added 3 h after initial infection. With CXM and CHL treatment, the PAO1 CFU data represent the sum of associated and invaded bacteria. A one-way ANOVA with Dunnett’s multiple comparison test was used to compare the intracellular CFU numbers at each time point after antibiotic addition (50 and 200 μg/mL) with those recorded at 3 h before addition of antibiotics and for each MOI used. All calculated P-values are shown in Supplementary Table S2.