Figure 1. Expression of follistatin in human tissues and breast cancer cell lines. (A) The expression of follistatin transcripts in different human tissues using RT-PCR. L is DNA ladder. 1-4 are placenta. 5-7 are ovary. 8 and 9 are normal breast tissues. 10 is omentum. 11 is liver. 12 and 13 are skin. 14 is colon. 15 is bone. 16 and 17 are breast cancer tissue. 18 and 19 are prostate cancer tissues. 20 is negative control. (B) Follistatin transcript splicing variants (FST344, NM_013409.2 and FST317, NM_006350.3) were detected in five breast cancer cell lines, i.e. MCF-7, MDAMB-231, ZR-751, BT-549 and Bt-20, using RT-PCR.
