Figure 3. FST overexpression model and functional assays. (A) FST344 expression plasmid produced a 3.5-fold increase in FST expression compared to pEF control plasmid in the transfected MCF-7 cells. (B) Invasion assay demonstrating a significantly reduced invasion rate in FST-overexpressing MCF-7 cells compared to control (p<0.001). (C) Percentage growth rate at day three of the assay, with significant increase in growth rate in FST overexpressing MCF-7 cells compared to control (p<0.0001). (D) This is maintained at day five of growth assay (p<0.0001).
