Figure 4.

Soluble calreticulin (CRT) induces macrophages spreading and migration. (A) THP1 macrophages were treated with 1 µg/ml of recombinant CRT, or not, for 24 h, and cell spread was evaluated. The spreading phenotype was confirmed by actin fibers labeling with phalloidin-TRITC. Characteristic differential interference contrast (DIC) and fluorescent images (DAPI/TRITC) are shown. Bar: 10 µm. (B) Wound healing scratch assays were realized using the J774 mouse macrophage cell line, in the presence or absence of CRT. The closure efficiency was evaluated as described in Section “Material and Methods.”