Figure 5.

(A) Macroglial staining of optic nerve sections with glial fibrillary acidic protein (GFAP; red) and DAPI for cell nuclei (blue). An unstructured GFAP immunoreactivity could be observed after I/R. (B) Evaluation of GFAP stained sections revealed no differences in GFAP⁺ area between the optic nerves of both groups. (C) On mRNA level, a significant up-regulation in GFAP expression could be shown in the ischemia group in relation to controls. (D) Additional staining of macroglia with vimentin (red). DAPI was used to label cell nuclei (blue). (E) No differences could be noted in vimentin⁺ area between both groups, but an increasing trend could be observed in the ischemic group. (F) Compared to controls, also on mRNA level, no differences in vimentin expression were measured in ischemic optic nerves. Scale bar: 20 μm. *p < 0.05.