Figure 2.

Enrichment of CD4⁺, CD4⁺CD25⁻, and CD4⁺CD25⁺ T cells from DA rats tolerant to Piebald Virol Glaxo rat strain (PVG) allograft. Lymphocytes from DA rats tolerant to PVG cardiac allograft were enriched for CD4⁺CD25⁺ T cells as in Section “Materials and Methods.” FACS profiles of unfractionated spleen and lymph node cells (top panel), and CD4⁺CD25⁺ (middle panel), and CD4⁺CD25⁻ (bottom panel) subpopulations. CD4⁺CD25⁺ T cell preparations were >85% CD25⁺ (middle left plot) and 70–80% expressed forkhead box P3 (FOXP3) (middle right plot). CD4⁺CD25⁻ T cell population had very few CD25^hi (bottom middle plot, <2%) or FOXP3⁺ (bottom right plot, <2%) cells.